Abstract: With the rapid development of fragmented learning, micro-lecture has raised great attention owing to its value features which are brief, short, simplification and micro. To utilize micro-lecture, the understanding of symbol elements of micro-lecture and their logical relationship is needed. As a result, from the perspective of semiotics, the research gives a deep interpretation of symbol elements of micro-lecture and their logical relationship, which represents the choice, the presentation and the illustration of teaching contents, and the interaction of micro-lecture design. In addition, the development of micro-lecture can realize the innovation of education system, the development of teacher's profession and comprehensive growth of students.