Abstract: The implementation of main responsibility is the key to the quality monitoring effect of collaborative education projects in universities, ultimately affecting the quality of talent cultivation. Research has found that there is a serious lack of main responsibility in the quality monitoring of collaborative education projects. By using interview and observation methods, analyzing organizations and individuals from the perspective of stakeholders, it is found that the lack of satisfaction of the interests and demands of various stakeholders is a practical dilemma of the absence of quality monitoring subject responsibility. The quality monitoring of collaborative education projects serves as a key tool for universities to improve teaching quality, with the ultimate goal of enhancing talent quality. All stakeholders should leverage their respective resource advantages to jointly assume monitoring responsibilities. The government should strengthen top-level design, establish and improve a long-term mechanism for collaborative education, and provide policy support and guarantees for stakeholders to play their roles; Enterprises should actively participate in the talent cultivation work of universities, integrate advanced concepts and technologies into university classrooms, and improve students' practical and scientific thinking abilities; Colleges and universities should establish a sense of quality culture, incorporate collaborative education quality monitoring into the entire teaching quality monitoring system, and form a quality management concept of full staff, full process, and all-round. Ultimately, a harmonious situation will be formed where the government provides support, enterprises provide assistance, universities connect, and multiple stakeholders jointly build, manage, and share resources, achieving win-win cooperation among multiple stakeholders.