Abstract: The purpose of this study was to explore the differences of vigilance of attention among college students with different trait anxiety levels. In this study, the state-trait anxiety questionnaire was used to investigate 160 college students, and the first 27% and the last 27% of the questionnaire scores were selected for the next experiments. In experiment 1, the classical attention network test (ANT) was used to explore the three subnetworks of attention. Experiment 2 focuses on the vigilance subnetwork test, and the program is changed to voice cue. In the first experiment, it was not found that the differences of the three Subroutine of the attention network test in the high and low trait anxiety group. In experiment 2, we found that the innate alertness level of high trait anxiety was higher than that of low trait anxiety. There was no difference in the response of the students with different trait anxiety levels to the phasic alertness. Students with different levels of trait anxiety responded more quickly to voice cues. In conclusion, the innate alertness level of high trait anxiety was higher than that of low trait anxiety. There was no difference in the response of the students with different trait anxiety levels to the phasic alertness.